| # | Title | Rating | Length |
|---|
| 1 | Nintendo Presents... | 2.75 | 0:01 |
| 2 | Opening Melody | 2.25 | 2:42 |
| 3 | Yoshi's Island | 2.75 | 2:11 |
| 4 | The Journey Begins | 2.5 | 1:01 |
| 5 | Practice Stage | 2.5 | 0:41 |
| 6 | Stage Start | 2.5 | 0:02 |
| 7 | Flower Field BGM | 3.5 | 2:52 |
| 8 | Goal! | 3 | 0:49 |
| 9 | Underground BGM | 3.5 | 1:45 |
| 10 | Castles & Forts BGM | 3.35 | 2:07 |
| 11 | Kamek's Theme | 3 | 0:30 |
| 12 | Stage Boss | 3 | 1:12 |
| 13 | Athletic BGM | 3.75 | 2:09 |
| 14 | Ground BGM | 3.25 | 1:30 |
| 15 | Yoshi Down! | 2.5 | 0:04 |
| 16 | Game Over | 2 | 0:08 |
| 17 | In the Boss Room | 2.75 | 0:57 |
| 18 | Big Boss BGM | 2.75 | 1:59 |
| 19 | Big Boss Clear | 2.5 | 0:07 |
| 20 | Map Screen | 3 | 2:13 |
| 21 | Bonus Challenge | 3.25 | 1:24 |
| 22 | Powerful Mario | | 0:58 |
| 23 | Bowser | 3.25 | 2:43 |
| 24 | Bowser Defeated | 2.5 | 0:08 |
| 25 | Rescuing Luigi | 2.75 | 1:30 |
| 26 | Ending | 2.5 | 3:55 |
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